Postpartum infection in perineal wounds is a problem during childbirth. The infection supports maternal mortality and morbidity in Indonesia 38%. The use of antiseptics to inhibit bacterial growth and prevent infection, namely 10% povidone-iodine has several side effects, one of which is allergic, thus encouraging research to find safer and more effective herbal ingredients. The research to prove the tannins on the leaves of star fruit (Averrhoa bilimbi leaf) as the inhibitor or the growth of bacteria S. haemolyticus α, β, S. aureus, and E. Coli. Subjects of the study were bacteria S. haemolyticus α, β, S. aureus, and E.coli. The design of the study was a completely randomized design, inhibitory test using disc diffusion method Kirby-Bauer with One Way ANOVA and Post Hoc Tukey HSD. The inhibitory power of bacterial growth S. haemolyticus, S. aureus and E. coli in tannins 1% and 10% are no better than iodine control 10%, in tannin 20% the growth inhibitory ability of bacteria were the S. haemolyticus α and S. aureus same as iodine control 10%, while S. haemolyticus β and E. coli were better than 10% iodine, namely S. haemolyticus α tannin 20% = 16.83 mm and control = 15.67 mm with p = 0.087 (> 0.05) not significantly different. S. haemolyticus β tannin 20% = 17 mm and control = 15.67 mm with value p = 0.035 (<0.05) was significantly different, S. aureus tannin 20% = 17 mm and control = 16 mm with p = 0.131 (> 0.05) was not significantly different and E. coli tannin 20% = 16.83 mm and control = 15 mm with a value of p = 0.002 (<0.05) was significantly different. The 20% tannin concentration is a significant concentration in inhibiting the growth of bacteria S. haemolyticus α, β, S. aureus, and E.coli so that it can be used as an antiseptic alternative to perineal wounds.